Cell Line Authentication Ppt

A few years after scientists working at johns hopkins established the first human cell line using cervical epithelial cells hela from cancer patient henrietta lacks in the early 1950s it was observed that vigorous lines could contaminate and overgrow slower growing cultures.

Cell line authentication ppt.

Applicants proposing to generate a new key biological and or chemical resource must describe the development and authentication of the resource in the approach section of the. Excel powerpoint audio or video files. Notice regarding authentication of cultured cell lines. Nih funding opportunities and notices in the nih guide for grants and contracts.

The str profiling method is a multiplex polymerase chain reaction pcr assay comprised of primers targeting 19 mouse str markers and two human str markers for interspecies contamination screening. In providing karyotyping information we can promote the advancement of biology projects. Thus the rigorous testing of cell cultures is inevitable. Cell lines are an invaluable research tool.

Notice regarding authentication of cultured cell lines not od 08 017. While dna fingerprinting techniques are used for the detection of individual human samples 11 12 and as an international standard for authentication of human cell lines 13 15 animal cell lines derive from genetically homogeneous lab bred individuals and require a different approach. 37 d925 d932 2009 cas. Cell line authentication is still poorly reported despite the prevalence of misidentification the calls for community action and the estimates of wasted research dollars 1 6 studies that are carried out with misidentified cell lines add misinformation to the literature are likely not to be reproducible.

Premature chromosome condensation pcc images human cells. A comprehensive approach to assessing the quality of human and nonhuman cell lines. Creative bioarray has performed many karyotyping banded chromosome analysis services for cell line authentication and some specific research. Cell line data base.

Do not include plans for the development and authentication of new key biological and or chemical resources such as a new human cancer cell line or a new antibody. Cell line identity and authentication. However while they are easy to grow and manipulate the risk of contamination is high. Since then we are using dna bases methods.

The consortium for mouse cell line authentication was formed to validate short tandem repeat str markers for intraspecies identification of mouse cell lines.